چكيده به لاتين
In this research, synthesis of carnosine (his-β-alanine) and porphyrin derivatives
[meso-tetrakis(4-trimethylanilinium)porphyrin and tetrakis(4-sulfonatophenyl)porphyrin] were investigated for comparing chelating ability of toxic metals such as Al3+, Cu2+, Hg2+ and Pb2+ in vitro. The reason for choosing these two compounds is that both of them are naturally present in biological systems and comparison of chelating ability of these two compounds has not yet been done. The molecular structure of the carnosine and porphyrin compounds was defined using different methods such as UV-Visible spectrophotometry, FT-IR, 1HNMR and LC-Mass spectrometry for carnosine. Comparison of The chelating property was investigated by UV-Visible spectrophotometer and voltammetry studies using differential pulse anodic stripping voltammetry (DPASV). Interactions of dipeptide, with various concentrations of metal ions show that, chelating ability of metal ions by chelator depends on several factors such as the size of the metal ion, molar ratio of metal to chelator, structure of chelator, charge density of the metal ion and the interaction time. In addition, cytotoxicity assays of metals and chelators were also performed on human lymphocytes, the order of the cytotoxicity of metals and chelators on lymphocyte was: Hg2+>Al3+>Cu2+>Pb2+ and TAPP>TPPS4>dipeptide. Furthermore the protective effect of chelators against cytotoxicity by toxic metals was investigated and results clearly indicated that the chelators reduced toxicity of applied metals in this cellular model. Observations and calculated data represented that all three chelators have high chelating potential to attract toxic metals according to the type of metal and appropriate dose of chelator, that can be used as suggested drugs in che chelation therapy.
Keywords: Chelator; Toxic metals; Carnosine; Porphyrin derivatives; Chemical kinetics; Voltammetry; Cytotoxicity.
