چكيده به لاتين
Abstract:
Magnesium-containing silicate bioceramics have recently gained much attention for tissue engineering applications due to their ability to stimulate cell proliferation and differentiation along with their adequate mechanical characteristics. Monticellite (33.33% of Mg) was chosen as an appealing biomaterial to identify time- and dose-dependent biocompatibility and in vitro osteogenic activity. In this research, high-pure monticellite ceramic (CaMgSiO4) nanoparticles were synthesized via a novel sol-gel method using ethanol and chloride ions for the acceleration of polycondensation process. The gel obtained from metal alkoxide and metal salts precursors dried at 100 °C; then, the dried-gel sintered at different temperatures to monitor the structural development of the final samples. The effect of various heat-treatment temperatures on the x-ray diffraction (XRD) patterns, followed by the calculations from scherrer’s equation, showed that the grain size of the synthesized powders at 1200 °C was around 28 nm. Based on dynamic light scattering (DLS) spectroscopy, the particles size distribution average was approximately 85 nm. Nanostructure hydroxyapatite (HA) with different percentages of monticellite nanobioceramic was applied to form new composites through the planetary ball mill, press and sintering at 1050 °C. After evaluating physicochemical characteristics of the synthesized bioceramics, apatite forming ability of the bioactive ceramics were investigated in simulated body fluid (SBF) after different time periods. A time- and dose-dependent MTT assay illustrated that monticellite promoted proliferation of bone like cell considerably more than positive and negative controls. The cell viability of the bioceramic was higher than HA (as bone like inorganic material) and control sample, demonstrating that cytocompatibility was promoted due to the increase of Mg content. The results of alkaline phosphatase (ALP) activity test demonstrated that the osteogenic proliferation of osteoblast-like G292 cell line enhanced more by the bioceramics extract than control and HA. Finally, the cytocompatibility of HA/monticellite composites was evaluated in different times and concentrations, that composite extract 90%HA-10%monticellite (concentrations 6.25 and 0.78 mg/ml) showed the highest cell growth and proliferation after 96 hours of cell culture.
Keywords: Magnesium, Sol-gel, Nanobioceramic, Bioactivity, Cytocompatibility
