چكيده به لاتين
Bone tissue engineering encompasses the principles of bone biology and engineering disciplines to augment bone loss through the use of temporary matrices called as scaffolds. Scaffolds are fabricated by materials belonging with various classes including polymers, ceramics. Chitosan (CS), is a natural biocompatible and biodegradable polymer, which has been widely used as one of the scaffolding materials.
Composite materials based on combinations of biodegradable polymers and bioactive ceramics, including CTS and HAp, are discussed as suitable materials for scaffold fabrication, although diopside is a member of bioactive silicates, because of its biocompatibility with host tissue and high mechanical strength, it’s a good choice for hard tissue engineering.
The scaffolds fabricated by freeze drying method, and the ratios of the ceramic components in composites were 5/57/38, 10/55/35, 15/52/33 (DP/HA/CS, w/w %) for purpose of using it for bone tissue engineering.
Chitosan and hydroxyapatite were extracted from shrimp shell and bovine bone respectively, and diopside nano particles prepared by sol gel method. The physicochemical properties of scaffolds were investigated using FTIR, XRD, SEM, EDX, techniques. Composite scaffolds had 76–92% porosities with interlinked porous networks. To investigate mechanical properties of samples, compression test was done. The data indicated that, sample with 5% diopside has the compressive strength in the range of cancellous bone compressive strength. Relative weight percentage and apatite formation of scaffolds after 4 week immersion in SBF solution showed that addition of diopside increases bioactivity of scaffolds and make them more stable in SBF solution. In order to evaluate biocompatibility of samples, MTT assay, cellular attachment and ALP activity tests were done. Results illustrated that the developed composite scaffolds could be a potential candidate for tissue engineering.
