چكيده به لاتين
The separation of suspended particles in the fluid flow or in the medical engineering field is the separation of cancer cells from the bloodstream or the separation of plasma from the blood and examples such as one of the important issues in this field. The analysis of blood components is an important diagnostic step in the diagnosis of the disease. As a result, the isolation of cancer cells from blood cells is critical to improving the accuracy and accuracy of diagnostic and therapeutic tests. The use of micro-fluid techniques can be considered as an effective way in this area, which has great functionality and accuracy. At the beginning of the research, the introduction and then the categorization of different isolation methods were presented and the hydrodynamic separation was selected in a specific way, and its operation, limitations, advantages, and problems were investigated. In the following, attempts have been made to review and present the articles, researches, and advances made so far in the isolation of cancer cells.
In the next step, the design of the chip is described in detail. This chip consists of two steps, the first stage of high-efficiency micromixer design and the second stage of the design of hydrodynamic filtration with a high percentage of arrests of particles is introduced. In this section, we have tried to discuss the overall design, which is discussed using a new design in numerical modeling, mixing process and improved isolation. The prominent features of this design include a small sample size, increased process speed, and the ability to control precisely the reaction environments.
In the next step, a variety of micro-scale fabrication methods have been investigated and a fabrication method with PDMS for chip has been selected. One of the advantages of this construction method is the significantly reduction in cost and construction time, the ability to build multiple chips with only one template, the multiplicity of the channel in the entire set, the possibility of embedding in microfluidic structures for use in a complete device, the possibility of creating geometries Different at the channel stage, it is possible to minimize microchannel up to 15 micrometers, and even in some places to 5 micrometers, PDMS transparency and the possibility of viewing events within the microchannel. Finally, the built-in chip for verifying and analyzing the data is done in a laboratory with a parametric study, which is described in detail.
