چكيده به لاتين
Tisuue engineering is combination of biology and material science to regenerate lesions that composed of cells, scaffolds and growth factors. Biodegradable scaffolds are one of the most important parts of tissue engineering.. Recently, in the field of biomaterials used in soft tissue scaffolds, scientists have turned their attention to the growth of natural polymers. Synthetic polymers are often hard and many are not biocompatible. On the other hand, natural polymers are biocompatible, but they are weak when used alone. The combination of natural and synthetic polymers provides the necessary properties for tissue engineering. In this study, gelatin was grafted to the polyurethane by modifying the surface by oxygen plasma. The process was initially made by polyurethane scaffolds by freeze drying with dioxan solvent, then placed in the oxygen plasma to improve the physical surface and activate the surface. After forming peroxide, the scaffold was immersed in a solution containing gelatin to form the bond. Then the tests were performed on the samples. Based on the results of electron microscopy (SEM), porous structure with randomly coupled pores was created in this method and the diameter of the pores increased from about 60-40 mμ to about 40-80 mμ. Infrared spectroscopy (FTIR), the presence of an amide type I and type II amide proved the gelatin bond with the polyurethane scaffold. The analysis of the water contact angle showed that the hydrophilic property of the scaffold after gelatin transplantation increased from about 250-100% over a period of 2 days to about 650-500%. Cellular behavior has shown that cell adhesion has improved in the gelatin-bonded scaffold, and cell proliferation can be easily observed; no signs of toxicity have been observed within 7 days. Based on the results of oxygen exposure, the placement of scaffolds can activate its surface activation and cause gelatin transplantation.
Achieving the physicochemical, mechanical and biological properties of the macromolecules used in this study can lead to the formation of an optimal new texture.
Keywords: Tissue Engineering, Scaffold, Surface Treatment, Plasma Oxygen, Polyurethane, Gelatin
