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In this research, the placement of nanofilms of Molecular Imperinted Polymer (MIP) Vitamin B2 (riboflavin) and the cephalexin-cadmium complex on the functionalized surface of the glass has led to the creation of stable sensors for spectrophotometric measurements of riboflavin and cephalexin- Cadmium Complex. In order to strengthen the strength of the MIP to the glass surface and provide stable sensors, first the glass surfaces were functionalized with three functional groups of amine, chlorine and epoxy, and the strength of the MIP was compared to the functionalized glass with each of these three functional groups. an‎d the best agent group that provided the highest bonding and adhesion strength was selected to replace glassy silanols. After functionalizing the surface of the glasses, uniformity of the nanolayer was obtained by checking the MIP solutions on the surface of the glass slides using a rotating coating system. After assuring the response of the sensors, effective parameters such as: type of washing solvent, volume ratio washing solvent, time staying in washing solvent, extraction solution pH and time staying in extraction solution, were optimized for both sensors. Then, the calibration curves were drawn in optimal conditions and suitability cultivars including linear range, sensitivity, detection limit and repeatability were investigated. In the final part of the study, the sensors used to detect and measure the cephalexin-cadmium complex and riboflavin in a capsule and human serum were used, which showed good recovery results.