چكيده به لاتين
Abstract:
Polycyclic aromatic hydrocarbons (PAHs) are persisted organic pollutant which have two or more fused benzene rings. They are widely distributed environmental contaminants that have hazardous biological effects, toxicity, and carcinogenicity. They originated from incomplete pyrolysis of organic compound. Some techniques such as adsorption, photolysis and volatilization are used in the determination of PAHs, which between these methods, adsorption is the best method. Micro-Solid phase extraction is a format of solid phase extraction. In this method, few milligrams of sorbent are packed inside a sheet of porous polymer membrane and edges of this sheet are heat sealed to convert it into a small bag.
In the present work, knitted polystyrene sorbent is prepared and was used as a new sorbent in µ-SPE method for extraction of PAHs in well water. Determination of analytes was performed by gas chromatography-mass spectrometry with electron impact detector.
To reach the optimum extraction efficiency, affecting parameters, including sorption and desorption time, amount of sorbent and volume of organic solvent were investigated by a central composite design. Optimum conditions were 10 minutes, 5 minutes, 27 milligram and 1 millilitre for mentioned parameters, respectively. Under optimum conditions the calibration curves in the range of 0.01-1000 ng/mL for naphthalene and acenaphthene, 0.001-1000 ng/mL for acenaphthylene and 0.1-1000 ng/mL for anthracene. The limits of detection (LOD) and limits of qualification (LOQ) were 0.3-10 pg/mL and 0.8-30 pg/mL, respectively. The relative standard deviation (RSDs) for four analytes at a concentration in 1 ng/mL were less than 11%. Recovery and enrichment factor were in 89-112 and 930-1120, respectively.
Keywords:
Micro-Solid phase extraction, Polycyclic aromatic hydrocarbons, Knitted sorbent, Polystyrene, Gas chromatography-Mass spectrometry
