چكيده به لاتين
Acidic Starch hydrolysis is not widely used today due to adverse side reactions and the impossibility of controlling reaction conditions, and enzymatic hydrolysis should be optimized due to the high price of the enzyme. Enzyme immobilization is a way to bump into the challenge of using the enzyme in the industry. Therefore, the project attempted to immobilize the alpha-amylase enzyme that is starch hydrolyzing on a covalent magnetic nanoparticle. After immobilization of this enzyme at 30 °C, 1000 ppm enzyme concentration, pH = 6 and 3 hours, optimum conditions for its use were investigated by various analyzes. Temperature, pH, initial enzyme concentration and appropriate time for hydrolysis were investigated. In the second phase of this study, attempts have been made to use this immobilized enzyme on magnetic nanoparticles for starch hydrolysis and the possibility and ability to reuse this biocatalyst. Initially, the highest concentration of starch was obtained, 5% by weight. The optimum conditions for the application of the immobilized enzyme in the starch hydrolysis were then evaluated in terms of temperature, pH, initial enzyme concentration, release rate during the hydrolysis, and the kinetics of enzyme adsorption. According to this study, the optimum temperature of 5% solution of starch hydrate was obtained at 40 °C and pH=6. The rate of enzyme release during starch hydrolysis was negligible and the absorption kinetics demonstrated the superiority of the immobilized enzyme in the starch hydrolysis. The possibility of enzyme reuse and enzyme stability was also investigated, with 40% of the immobilized enzyme activity retained after 6 cycles and only 20% of it released after 15 days.
Keywords:
Alpha-amylase enzyme, magnetic nanoparticles, covalent immobilization, starch hydrolysis
