چكيده به لاتين
Background: Early detection of cancer will increase the probability of treatment effectiveness and prevent severe disease. The main challenges of cancer diagnosis are speed, cost, and accuracy. One of the diagnostic methods of cancer is the use of Point-of-care (POC) method that can be used to screen a population for initial diagnosis. The purpose of this study is to design a POC diagnostic platform for breast cancer that could respond either positively or negatively to a patient with precise accuracy in a short time and at low cost. Material and methods: In this study, first, a cheap and reproducible screen-printed electrode based on Printed circuit board (PCB) technology and physical vapor deposition was designed and tested, then using the Taguchi method optimized the graphene-based biosensor platform for cellular biomarkers and molecular detection. Following the development of PCB-based graphene-polyaniline biosensor, this platform was evaluated in HER2 + breast cancer cells using the Trastuzumab antibody and ascorbic acid assay. Results: Briefly, in the screen-printed electrode fabrication section, higher stability, good potential for using in variety of applications, and providing unique technical properties (e.g., wide working voltage window and high current gain), as well as, low cost and uncomplicated method fabrication are noteworthy. In the PCB-based biosensor platform fabrication, we detected cancer cells with linear range between 10 to 200 cells and ascorbic acid with 10 uM detection limit. Conclusions: The outcome of the fabrication and characterization steps indicates the successful implementation of the electrochemical platform for Point of Care diagnostic based on PCB technology. Due to its accessible and inexpensive preparation capability, this platform can be easily used for early detection and inexpensive screening of large populations. The platform also has the potential to be customized for other diseases and biomarkers.
