چكيده به لاتين
In this work, magnetic molecularly imprinted polymers (MMIPs) were applied to separate human insulin. Electrostatic functional monomers (EFMs) contain acrylamide (AAm) as neutral-hydrophilic, alongside with methacrylic acid (MAA) and 2-dimethyl aminoethyl methacrylate (DMA) were chosen as electrostatic functional monomer. N, N-methylene bisacrylamide (MBA) as a cross-linker was used in the magnetic imprinted polymer of insulin (as template molecule) onto vinyl-coated magnetic particles. The MMIP was prepared with different ratio functional monomers in two cases of hydrophilic-hydrophobic-neutral (MMIP3: at four levels) and hydrophobic-neutral (MMIP2: at three levels) toward insulin. The adsorption experiments were performed under the optimization condition. The results were shown that the MMIPs had high adsorption capacity, good selectivity, and fast adsorption kinetics to insulin. The kinetic results showed that adsorption in both samples follows the chemisorption adsorption mechanism. The maximum adsorption (Q, mg.g-1) and imprinting factor (IF) of MMIP3 and MMIP2 were found 67 mg.g-1, 3.0, and similarly were achieved 54 mg.g-1 and 4.1. Besides, the relative selectivity coefficient for MMIP3 and MMIP2 was obtained 4.39 and 27.82, respectively. The composition and characterization of synthesized particles were studied by Fourier transform infrared spectrometry (FT-IR), dynamic light scattering (DLS), and the thermo-gravimetric analysis (TGA). Meanwhile, vibrating sample magnetometer (VSM) was carried out to characterize the magnetism of MMIPs.
Keywords: Magnetic molecularly imprinted polymer, Magnetic particles, Insulin, Electrostatic functional monomers
