چكيده به لاتين
DNA sequencing is an accurate technology for detecting the four bases of adenine, cytosine, guanine and thymine in a DNA molecule, which is performed in different ways. The latest method that is currently being studied is nanoporous sequencing, called the third generation sequencing technique, which aims to increase throughput, reduce costs, time and consumables. Nanopores consist of three categories: biological, solid state and hybrid. In this paper, using molecular dynamics simulation, four strands of DNA including A9, C9, G9, and T9 pass through a solid Si3N4 nanopore with a thickness of 12 and A diameter of 4 nm was examined by applying a voltage of 20 volts. Given that the biggest challenge facing researchers in the passage of DNA is its high speed in the passage of nanopores, so the main purpose of this paper is to investigate the effect of concentration on the passage time and speed of DNA through the nanopore. The nanopore along with the DNA was placed in magnesium chloride solution at three concentrations of 0.5, 1 and 2 M and by applying a voltage of 20 volts to both ends of the hole and forming an electric field inside the nanopore, conditions were provided for DNA to move inside the nanopore. . Simulations performed by NAMD software showed that as the concentration of the solution increased, the velocity of DNA within the nanoporous decreased. These results were consistent with similar cases previously studied in the literature. In addition to studying the movement of DNA within the cavity, the behavior of chlorine and magnesium ions was also examined.
