چكيده به لاتين
Cancer is one of the leading causes of death worldwide; cytotoxic drugs are used to treat cancer; these drugs have many adverse effects on the patient and kill both healthy and pathogenic cells. Metal-organic frameworks as carriers of chemotherapeutic drugs are of great importance in drug delivery due to their unique properties such as high porosity, improved pharmacokinetics, good stability against high temperatures and various chemical environments, and controlled release. Synthesized mesoporous nanoparticles based on metal-organic structures have a remarkable ability to load and encapsulate high doses of drugs. Cell-penetrating peptides as a new class of peptides also have a great potential for transmitting therapeutic agents into the cell that can help treat challenging diseases such as drug-resistant cancers. In this study, preparation and characterization of drug-carrier nanoparticles, made of metal-organic frameworks (with porous structure), spherical and magnetic iron nanoparticles, doxorubicin (as an anti-cancer agent), and cell-penetrating peptide are well done and confirmed by a variety of analysis methods. To increase the entry into the cell of Fe3O4@Fe-MIL-101-NH2 magnetic nanoparticles containing doxorubicin, a cell-penetrating peptide consisting of the amino acid sequence of arginine-tryptophan-arginine-tryptophan-arginine-tryptophan-cysteine synthesized in the solid phase and was attached to the linker in the organic-metal structure through an amide bond. The peptide helps to increase the entry of nanostructures into the cell due to its positive surface charge as well as the appropriate hydropathy index. In order to confirm the structure of the synthesized MOF, several analyzes such as FT-IR, EDX, TGA, FE-SEM, XRD, and VSM were used. Mass spectrometric analysis was also used to confirm the structure of the cyclic and linear peptides. Keywords: Metal-organic framework, Iron oxide nanoparticles, Cell-penetrating peptides, Solid phase synthesis, Controlled release
