چكيده به لاتين
Silk fibroin can be used in tissue engineering, wound dressing, artificial blood vessels, surgical sutures, and many other biomedical applications due to its good reactivity, excellent biological compatibility, and remarkable mechanical properties, biodegradability, blood compatibility, and good water permeability. Pure silk films, on the other hand, have little mechanical strength in dry conditions and can't be utilized as a wound dressing, so they're frequently mixed with other polymers like sodium alginate to improve their qualities. Natural origin, good hydrophilicity, excellent biocompatibility, biodegradability, and low cost are some of sodium alginate's distinguishing characteristics. For electrospinning under controlled conditions, purified fibroin was dissolved in formic acid with an optimal concentration of 16% W/V. Then silver nitrate was added in 0.5 and 1%wt of the polymer solution. For electrospinning, sodium alginate (2% w/v) and polyvinyl alcohol (7% w/v) solutions with a 3: 7 ratio were created. Finally, fibroin nanofibers containing silver nitrate, sodium alginate, and polyvinyl alcohol were spun using two distinct nozzles in a combined or continuous electrospinning process. By using a green synthesis method, silver metal nanoparticles were made. To cross-link nanofibers and boost their stability in aqueous media, citric acid and methanol were used. Infrared spectroscopy (FTIR) was used to examine the chemical structure of the nanofibers. SEM and FESEM at specified scales were utilized to evaluate the scaffolds' morphology and structure, while EDX and MAP were employed to confirm the presence of silver nanoparticles. Antibacterial and cytotoxicity analyses were performed to determine the potency of gram-positive s.aureus bacteria and cell viability. Antibacterial testing was carried out by using the MIC and MBC procedures, as well as a disc diffusion test. The swelling rate and vapor permeability tests, which are important components of assessing the suitability of a scaffold for wound dressing, were also performed and the results were reported in the fourth section. Cell adhesion was assessed using contact angle analysis, while cell adhesion and morphology were assessed using FE-SEM imaging. Contact angle analysis was used to determine cell adherence, and FE-SEM was used to determine the morphology of adhering cells. An ICP test was also carried out to assess the release of silver metal nanoparticles during a 48-hour period. UV-visible analysis was also used to figure out how much vitamin C was wrapped up in SA-PVA-CA nanofibers.
