eva‎luation of Targeted Delivery of Nanobody-coated Silver Nanoparticles to Vascular Endothelial Growth Factor (VEGFR2) Receptor of Breast Cancer Cell Line

9/20/2022 12:00:00 AM

A new nanocomposite developed to target silver nanoparticles as a drug for tumor cells and to create a new anti-cancer agent. Albumin-coated silver nanoparticles (ASNPs) were synthesized and after binding to nanobodies were assigned against the vascular endothelial growth factor receptor for their anti-cancer effects against MDA-MB 231, a human breast cancer cell line. ASNPs synthesized were eva‎luated by various methods including UV / visible spectrometry, DLS, ELS and SDS-PAGE. Specific antibody fragments against vascular endothelial growth factor receptor after expression in Escherichia coli were purified by affinity chromatography and used for covalent binding using NHS-EDC to synthesized ASNP. The manner of binding was investigated by examining changes in charge and zeta potential by DLS method and also by examining changes in molecular weight of proteins by SDS-PAGE method. It was found that the size of silver nanoparticles after binding to albumin (ASNPs) was 170 nm and had a negative charge with a zeta potential of about -17 milli volts, which increased in size and decreased the zeta potential compared to silver nanoparticles alone. Also, the results of visible ultraviolet spectroscopy of a specific peak at a wavelength of 240 nm in nanoparticles coated with albumin compared to single nanoparticles have been produced, which indicates the coverage of albumin on silver nanoparticles. After covalent bonding with a specific nanobody against blood vessel endothelial growth factor, the size was changed to 742 nm and to a zeta potential of about -10 milli volts. The results of SDS-PAGE confirmed the covalent binding of the antibody fragment to the albumin-coated nanoparticles, which increased the molecular weight to about 80 kDa. eva‎luation of the anti-proliferative effects of this nanocomposite on MDA-MB 231 cells compared to HEK293 control cells showed the improvement of the anti-cancer properties of this structure. The results of this study show that ASNPs are characterized by antibody fragments that can be targeted for the treatment of cancers.

سرطان , نانوبادي , آلبومين , بيوكانجوگاسيون , نانوذره نقره

Cancer , Bio-Conjugation , Albumin , Silver Nanoparticle , Nanobody

Seyyed Amirmahdi Mafakherian

Hossein Ghafuri