گلزار راغب

Piroxicam is a pain reliever and anti-inflammatory drug from the group of non-steroidal anti-inflammatory drugs. Recent research finds it necessary to design new drug delivery systems, especially nanocarriers, to achieve the best drug performance. For this purpose, in this research, PRX-HAp@BSA nanoparticles (BHD) were synthesized for the sustained and controlled release of piroxicam to improve the effectiveness of the drug. In order to compare and eva‎luate this system, PRX-HAp nanoparticles (HD) and PRX-BSA nanoparticles (BD) were also prepared and synthesized. In order to identify the synthesized nanoparticles and to check and confirm the existence of a bond between the carrier nanoparticles and the drug, Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction Crystallography (XRD), Differential Scanning Calorimetry (DSC) and UV-visible spectroscopy, which showed the formation of the carrier nanoparticles, the physical absorption between the carrier nanoparticles and the drug and also the presence of the drug (while maintaining the effectiveness). In addition, Field Emission Scanning Electron Microscope (FESEM) and ZetaSizer (ZetaSizer) tests were performed, which showed that the nanocarrier (BHD) has a uniforms particle size distribution with a spherical morphology (198±1.47) nm and an average hydrodynamic diameter (261) nm, and compared to the other two nanocarriers (BD) and (HD), it has a more equivalent zeta potential value. The drug release test and the investigation of the release mechanism of all three mentioned systems were performed at pH 7.4 and 4.04 and the Free Drug (FD) at pH = 7.4, the drug release of the nanocarrier (BHD) compared to (FD) and two nanocarriers (BD) and (HD) in the environment with both pH, are released at a lower speed, which slow release of the drug is desirable for controlled and stable drug delivery, also the absence of explosive drug release in the samples indicates the loading of the drug in the nanoparticles. The drug release mechanism of all samples follow the Korsmeyer-Peppas equation and their penetration is non-Fickian. Finally, Cell Proliferation and Survival (MTT) Assays for all three systems were eva‎luated for 48 hours on the RAW 264.7 cell line compared to the positive control (piroxicam (D) Drug) and negative control (RAW 264.7). The cells showed higher survival against nanocarrier (BHD) than the other two nanocarriers (BD) and (HD) and Drug (D).

پيروكسيكام- رهايش دارو- نانوذرات- آلبومين سرم گاوي- هيدروكسي آپاتيت.

Piroxicam- Drug Release- Nanoparticle- Bovine Serum Albumin- Hydroxyapatite.

Golzar Ragheb

Dr. Jafar Javadpour - Dr. Maryam Saeidifar