سهند ذبيح غلامي

Melt-derived bioactive glasses in the system of SiO2-Na2O-CaO-P2O5-Bi2O3 were prepared. The effect of manganese, and copper (1.9 mol.%) were investigated individually and together in points of optical and biological views. UV/vis/NIR Absorption spectroscopy indicated a broad absorption band around 800 and 400 nm for the copper- and manganese-doped glasses, respectively. In photoluminescence (PL) spectroscopy, which was also performed for the samples excited at 750 nm, an emission band at 823 nm was appeared. Furthermore, glass samples exposed to 808 nm wavelength showed a rise in temperature, so that glasses that showed a more absorption in the NIR range also showed a more temperature rise. The glass degradation rate in phosphate-buffered saline (PBS) was measured using inductively coupled plasma optical emission spectroscopy (ICP-OES). The results indicated that both the manganese and copper can decrease the degradation rate when compared to the base glass (G1). Moreover, while the copper ion bearing samples showed a higher photothermal activity, the presence of manganese enhanced this property as well as the number of osteoblast-like cells, according to MTT assays. On the other hand, after irradiating with an 808-nm laser beam (5 W/cm2) for 1 min, the viability of MG-63 cells cultivated with 500 µg/ml of manganese- and copper-containing sample (G4) was reduced dramatically to about 40% as a result of heat generation through PT effect. Finally, the bioactivity of the optimum sample was eva‎luated by soaking the bulk glass in simulated body fluid (SBF). Images of field emission scanning electron microscopes (FE-SEM) coupled with energy dispersive spectroscopy (EDS) analysis proved the formation of hydroxyapatite-like precipitation on the surface of the glass sample.

شيشه زيست‌فعال , درمان به روش فوتوترمال , ترميم استخوان , مس , منگنز

Bioactive Glass , photothermal therapy , bone regeneration , copper , manganese

Sahand zabih Gholami

Dr. Eftekhari _ Dr. Tajabadi