ارمان مومني

Wound healing is a complex and dynamic process that accelerates and improves it by preparing a suitable environment. Designing a suitable substrate that has properties similar to the skin and can provide the possibility of cell growth is one of the most important external factors effective in the wound healing process. excellent and remarkable mechanical properties, biodegradability was used as the main material in the direction of electrospinning in the ratio of 6:2:2 ml. The purpose of this research is to make polyvinyl alcohol-gelatin-albumin scaffold using electrospinning method, which was loaded on polyvinyl alcohol-gelatin-albumin nanofibers with concentrations of 1 and 3% of zinc oxide nanoparticles. In order to create a transverse link and increase the stability of the electrospun scaffolds, glutaraldehyde vapor was used in the amount of 20% by weight of the polymer solution. By controlling the electrospinning parameters and morphological studies, it was determined that uniform nanofibers without willows with an average diameter (432 ± 39-496 ± 43 - 405 ± 36 - 450 ± 34 - 506 ±48) were obtained. The presence and proper distribution of zinc oxide nanoparticles was confirmed by elemental analysis and MAP. In the swelling test, the samples reached 6.5 to 8 times their initial weight, which indicates the high absorption of nanofibers. In the permeability test, the samples showed numbers between (1820 to 2103), which is a suitable rate for a wound dressing to create a suitable micro environment for wound healing, as well as the results of the antibacterial disk diffuse test with a halo diameter of 7 mm indicates the antibacterial ability of nanofibers containing 3% by weight of electrospun nanoparticles against gram-positive bacteria S. aureus (S. aureus) and the diameter of the non-growth halo is 10 mm against the gram-negative bacteria Escherichia coli (E.coli) using MIC-MBC analysis with 1/2 and 1/4 dilutions for gram-positive and Dilutions of 1/4 and 1/8 for gram negative were proved as a supplementary test of this result. To eva‎luate the cell viability, the MTT test and L 929 fibroblast cell line were used, which was done indirectly and according to ISO10993-5 in different concentrations for 48 hours, and the cell viability was within the desired range, to eva‎luate The amount of zinc element released during 48 hours of ICP analysis was performed and the concentration of zinc ions in PBS solution was reported as 0.73 ppm for nanofibers containing 1% by weight of nanoparticles and 1.16 ppm for containing 3% by weight of nanoparticles. As a result, according to the obtained results, it can be concluded that the designed structure is suitable as a wound dressing, besides not causing toxicity, it shows good antibacterial properties. In addition, this structure can be achieved with a simpler, faster and cheaper method than other methods.

پلي ونيل الكل , ژلاتين , آلبومين , مهندسي بافت , زخم پوش , آنتي باكتريال , نانوذرات اكسيد روي , الكتروريسي

polyvinyl alcohol , gelatin , albumin , tissue engineering , wound dressing , antibacterial , zinc oxide nanoparticles , electrospinning

Arman momeni

Dr. Alireza khavandi