چكيده به لاتين
Despite the prevalence of cartilage injuries such as arthritis, this tissue has a low ability to repair itself due to its inherent properties. The current methods of treating these diseases are associated with many problems, so finding an alternative method using nanotechnology and tissue engineering is a promising solution. In this research, an attempt has been made to prepare an engineered cartilage scaffold to repair this tissue so that it can replace cartilage in terms of physicochemical, mechanical, and biological properties. For this purpose, for the first time, the human amniotic membrane has been used in making this hydrogel.
At first, based on previous studies, volume concentrations of 5, 10, 15, and 20% of amnion solution were selected, and concentrations of 0.1, 0.25, 0.5, 0.75, and 1% of genipin by weight were used to obtain hydrogels. According to the observations of water absorption and degradation tests, the concentrations of these materials were optimized. Then, SEM and FTIR tests were performed to characterize the prepared scaffolds. Next, by analyzing the mechanical and biological properties of the scaffolds, the percentage of optimal composition for gelatin hydrogel scaffold containing amniotic membrane, 10% gelatin/10% amniotic membrane, and 1% genipin was obtained. The results of these tests showed that the synthesized scaffold can withstand compressive stresses of 0.1-0.2 MPa without breaking or disintegrating. At the same time, the scaffolds have a suitable environment for the growth and proliferation of mesenchymal stem cells, and the addition of the amniotic membrane has increased the proliferation of cells compared to the scaffold without it.
In the second phase of the project, by characterizing the mesoporous silica nanoparticles using FTIR, SEM, and XRD tests, it was found that the synthesized nanoparticles are suitable for loading and transporting the drug dexamethasone. Then the standard graph of dexamethasone was drawn and its loading capacity and efficiency on silica nanoparticles were checked, after choosing the optimal ratio of silica:dexamethasone, weight ratio 1:10, porous silica nanoparticles containing dexamethasone were loaded in the optimal scaffold of the first phase of the research and its cell growth was checked by MTT assay. According to the results of the present study, it can be said that the prepared scaffold is a promising method for repairing damaged cartilage tissue.
