سمانه حيدري

In this study, we investigated the effect of tannic acid as a type of hydrogel cross-linker with percentages of 1,10 and 20% on the structure of hydrogel prepared based on carboxymethyl chitosan and dopamine. This eva‎luation examines the changes made in the hydrogel from the point of view of biological, physical, mechanical and chemical characteristics. The chemical structure of the hydrogel was confirmed by infrared spectroscopy (FTIR). Cytotoxicity and DAPI analysis were performed to find out the viability of the cells and their presence was observed in the hydrogel, and their number increased with the tannic acid concentration. The presence of tannic acid in the structure of hydrogel, was effective in the increase in cell viability. Scanning electron microscope (SEM), showed a decrease in the pore size with the increase in the of tannic acid content. The results of rheology test, showed an increase in the storage modulus (G′) and the loss modulus (G′′) values with the increase cross-linker content in the hydrogel structure. There was also an increase in the viscosity with the increase in the tannic acid concentration. The results of the degradation and swelling test showed that hydrogels without cross-linking agents have high swelling and degrade in a shorter period of time compared to the samples containing cross-linkers. on the other hand, with the increase of tannic acid as an agent. Therefore, the use of tannic acid as cross-linker was effective in improving the properties of hydrogel and thus it can be considered as a good alternative green cross-linker.

هيدروژل , شبكه ساز , كربوكسي متيل كيتوسان , پلي اتيلن گليكول , اسيد تانيك , زيست سازگاري , زيست تخريب پذيري

hydrogel , cross-linker , carboxymethyl chitosan , polyethylene glycol , tannic acid , biocompatibility , biodegradability

samaneh heidari

Dr khavandi- Dr javadpour