چكيده به لاتين
One of the most common types of cancer is ovarian cancer, for which different treatment methods such as surgery, chemotherapy, use of anti-angiogenic drugs, radiation therapy, hormone therapy and gene therapy have been used. Among the basic problems in cancer treatment is drug resistance, which can be solved by using combination therapy instead of monotherapy methods. In this regard, the simultaneous use of drugs and gene therapy is an interesting way. Curcumin is one of the natural medicines derived from turmeric, which has low toxicity, plus high safety and accessibility. This substance is able to suppress and treat a large number of malignancies, including cancer. On the other hand, miRNA34-a is one of the effective genes on P53 gene and its use leads to programmed cell death or apoptosis of tumor cells. As a result, the combination of the mentioned drug and the gene seems to be an effective combination for treatment. Of course, there are challenges for the delivery of these therapeutic substances, such as gene breakdown and the instability of the curcumin hydrophobic drug in the bloodstream. To solve this type of problems, the use of drug carriers in nano dimensions is a useful solution; In this study, Niosomal nanoparticles were used to investigate the therapeutic effects of the mentioned items on ovarian cancer cell line or OVC3R.
The formulation of niosomes was optimized in terms of the percentage of constituent compounds in order to achieve the best results in the field of cell absorption, drug loading, nanoparticle stability, controlled release and particle size. In addition, in this study, the effect of these therapeutic carriers on increasing the expression of the P53 gene and inhibiting the Nf-κB gene and the apoptosis amount of OVC3R cells and of course their cytotoxicity were investigated.
