مرضيه مطيعي پور

In this study, electrospun scaffolds based on polyol lactic acid/polyethylene oxide (PLLA-PEO) containing mesoporous titanium dioxide-alendronate (AST-TiO2) nanoparticles, loaded with the drug dexamethasone (DEX) with the ability to be used were prepared in bone tissue engineering. For this purpose, pure mesoporous titanium dioxide (TiO2) nanoparticles and AST-TiO2 nanoparticles were first synthesized. According to the obtained results, the size of AST-TiO2 and TiO2 nanoparticles was obtained in the range of 15-30 nm. According to the results of the BET test, the presence of alendronate increased the specific surface area of nanoparticles from 2.365 m2/g for TiO2 nanoparticles to 1.520 m2/g for AST-TiO2 nanoparticles. This increased the amount of drug loading in AST-TiO2 nanoparticles by 43.16% compared to TiO2 nanoparticles by 17.36%. Also, 78% and 47% release rate of DEX drug from AST-TiO2 nanoparticles were observed at pH 5.5 and 7.4, respectively, after 96 hours. On the contrary, TiO2 nanoparticles with the highest drug release rate did not show any change in the drug release rate with respect to pH changes. In the following, after the preparation of PLLA-PEO polymer microfibers with and without nanoparticles by electrospinning method, the morphology and mechanical properties of the fiber network were eva‎luated. The results showed that the presence of AST-TiO2 nanoparticles in the scaffold (fiber network) caused a decrease in the average diameter of the fibers. The tensile strength of scaffolds made by adding 5% by weight of nanoparticles increased by 8.5% compared to scaffolds without nanoparticles. The results of in vitro studies on mesenchymal stem cells derived from human fat showed that in addition to the appropriate biocompatibility of the prepared scaffolds (based on the MTT test), the scaffolds containing nanoparticles modified with alendronate have a higher bone differentiation capacity. compared to the control group. Therefore, the scaffold introduced in this study can be used as a potential candidate in bone tissue engineering applications.

مهندسي بافت استخوان؛ الكتروريسي؛ داربست پلي ال-لاكتيك/پلي اتيلن اكسيد؛ نانوذرات مزوپور تيتانيوم دي اكسيد-آلندرونيت؛ دگزامتازون؛ حساس به pH

bone tissue engineering; electrospinning; poly-L-lactic/polyethylene oxide scaffold; mesoporous titanium dioxide-alendronate nanoparticles; Dexamethasone; pH sensitive.

marziyeh motiei pour

mohamad reza moghbeli