چكيده به لاتين
Flaxseed is a notable source of polyunsaturated fatty acids (PUFA) like omega3 alfa linolenic acid (ALA) and omega6 linolenic acid (LA). This study involved extracting flaxseed oil using Cold-press Extraction (CPE), Ultrasound-Assisted Extraction (UAE), and Mixer-Assisted Extraction (MAE) methods, employing n-Hexane as the solvent with maximum extraction yield of 62.73%, 94.56%, 95.45%, respectively. GC-FID analysis was utilized to determine the composition of the extracted flaxseed oil and quantify the content of PUFA obtained for CPE, UAE, and MAE, resulting in values of 57.2%, 66.6%, and 66.3%. The iodine values for CPE, UAE, and MAE were 139.83, 182.74, and 175.73 (g of I2/100 g of oil), respectively, used to qualify the presence of unsaturation bonds. For the analysis of UAE and MAE data, a Quadratic model within the framework of RSM was employed. The regression coefficient (R2) exceeded 99% for both approaches. The optimized parameters were determined for UAE (t=42.0019 min, PS=0.282 mm, P=74.95 W) and MAE (t=59.64 min, RM=2959 rpm, PS=0.274 mm, T=20.28 °C). Specifically, in the UAE method, the Power parameter held the lowest p-value, while in the MAE method, Particle size exhibited the highest F-value and the lowest p-value, indicating their notable influence on the extraction yield within the mentioned methods.