امين حيدري پهراباد

Biopsy is the best method of cancer diagnosis and the most important sample that is isolated and monitored in liquid biopsy; Isolation and direct counting of CTC from patient's blood as a non-invasive approach for cancer diagnosis. The current research aims to improve and complete the recently presented microfluidic separation methods and aims to design and fabrication a microfluidic chip with the possibility of accessing the cells after the separation process and maintaining the biological conditions of the cells in order to perform additional studies and also with simple geometry and accuracy. After study various manufacturing methods, design and simulation (Comsol software) and geometric and process optimization (Design Expert software), a microchip was implemented as the simplest microfluidic chip for separating micron particles and then manufactured it with molds made by micromachining and a cutter plotter with a width of 500 μm and the height of the channel was 90 μm. Further, by preparing cells in the laboratories of the Pasteur Institute and conducting experiments in the University of Science and Technology, the performance of the microchip was eva‎luated and the isolation of MCF7 cancer cells and mouse fibroblast L929 (instead of WBC cells) with sizes of 20-25 and 12-15 μm with separation of about 89% and maintaining the natural conditions of the cells were shown, and millifluidic concepts and wall separation were introduced in the separation of micron. Also, other innovations such as combining microvortex and centrifugal methods for microfluidic separation, providing a method to reduce the effects of Dean flow in centrifugal microchannels to improve separation, and providing an expansion centrifuge microchannel with a sub-controller output were presented in this research.

ميكروسيالات , سلول سرطاني در گردش , ميكروساخت , جداسازي ميكروفلوئيديكي

microfluidics , circulating tumor cell , Microfabrication , microfluidic separation

Amin Heidari

Dr. Mehdi Moghimi