eva‎luation of the behavior of alginate hydrogels against calcium-based crosslinkers and silver nanoparticles using QCM-D nanosensor

11/20/2024 12:00:00 AM

QCM-D is a very sensitive technique for determining mass change in tens of nanograms or mass density less than 1 μg/cm2. Simplicity in concept, high sensitivity in mass detection, low costs and easy operation are the advantages of QCM-D nanosensor. Alginate or alginic acid is a suitable choice for medical applications such as tissue engineering, wound dressing and drug delivery due to its biocompatibility, swelling behavior, low costs and loading capacity. Ca2+ ion is one of the most widely used cation for cross-linking sodium alginate. In this research, sodium alginate hydrogel was prepared in 6 different concentrations. Then, in the first phase of experiments, two conventional calcium crosslinkers, calcium chloride and calcium nitrate, were prepared in four different concentrations. Four different protocols were designed and discussed to perform the experiment. Crystals, deionized water and all the prepared materials were once pure in different repetitions placed on the quartz crystal microbalance nanosensor with dissipation monitoring (QCM-D) to measure the accuracy and repeatability of the crystals. The interactions between the alginate hydrogel and its crosslinkers were measured in three replicates using the QCM-D nanosensor in situ and according to the selected protocol. In the second phase of experiments, silver nanoparticles were synthesized and purified by bottom-up method. Synthesized nanoparticles were monitored by dynamic light scattering analysis, field emission scanning electron microscope and X-ray energy diffraction spectroscopy. The size of silver nanoparticles was 64.9 nm with a suitable distribution based on the results obtained from dynamic light scattering (DLS) analysis. Silver nanoparticles were prepared in three different concentrations. Then, their interaction with sodium alginate hydrogel was measured using QCM-D nanosensor. The UV-Vis absorption spectrum of all materials used, including alginate, calcium chloride, calcium nitrate and silver nanoparticles, was measured by spectrophotometry. All the tests were performed with the help of the commercial ELISA device repeated in three repetitions and the results were compared with the results obtained from the discussed QCM-D nanosensor. Also, to observe the effect of different concentrations of calcium-based crosslinkers on alginate hydrogel, SEM images were prepared from them at different times. In addition, SEM images of commercially available quartz crystals were prepared and compared.

نانوسنسور QCM-D , ميكروباالنس كريستال كوارتز با پايش اتالف , هيدروژل آلژينات , كراس لينكرهاي كلسيمي , نانوذرات نقره

QCM-D nanosensor , Quartz crystal microbalance with dissipation monitoring , alginate hydrogel , Calcium-based crosslinkers , Silver nanoparticles

Mohammad Nooshnab

Mohammad Nooshnab