چكيده به لاتين
Omega-3 has been introduced as a widely used medicinal and food supplement in the world. For many years, fish was used to produce omega-3, and these products are of low quality and high price. Microalgae has been introduced as a new source of omega-3 globally, and the advanced countries of the world have taken advantage of microalgae and industrial production of omega-3 in the last decade.
In this research, the main goal is to experimentally investigate the production of omega-3 from microalgae so that it can be produced as a commercial product in the country in the future. Among the microalgae that have the ability to produce omega-3, nanochloropsis microalgae was selected as a suitable strain for the purpose of producing EPA, which is one of the important types of omega-3, and was used in the research. In this research, the parameters affecting the growth and production of EPA have been identified, which include light, carbon, nitrogen and phosphorus. The investigation of the mentioned parameters has been carried out in two ways, by applying changes at the beginning of the cultivation and creating additional food stress in the middle of the cultivation, both of which have shown positive and negative results in the production of EPA. Microalgae cultivation is continuous in the time intervals of 14 to 24 hours. The days depend on the type of cultivation. Microalgae fat was also extracted using Bligh & Dyer protocol and using methanol-chloroform solvent, and the obtained EPA analysis was done using spectrophotometric device and measurement of absorption rate. The investigation of carbon and phosphorus parameters in the research has had a positive effect, which has led to the production of more EPA than other investigations. In the investigation of the effect of phosphorus at the beginning of the cultivation and under the title of additional phosphorus stress in the middle of the cultivation, the highest amount of EPA occurred in the cultivation with a high percentage of phosphorus, which is equal to 134.8 mg in a four-liter culture, which brought 3.241 grams of biomass. In the studies of carbon effect, the highest amount of EPA was obtained by applying the maximum concentration of carbon in the middle of the culture, which is equal to 156.9 mg in a four-liter culture. The highest amount of biomass in the carbon study is also equal to 3.205 grams. However, in the investigation of the effect of nitrogen and adding additional nitrogen to the culture, no significant positive effect was observed in terms of increasing the amount of fat and EPA production, so that the highest amount of EPA is equal to 112.9 mg, but the highest amount of biomass has been obtained in this study, which is equal to with 3.242 grams. The application of additional carbon and phosphorus in cultivation has resulted in a 39 and 20% increase in EPA production, thus, in the final summary of this research, the use of additional carbon and phosphorus in nanochloropsis microalgae culture can be introduced in order to produce more omega-3, especially EPA.
