چكيده به لاتين
Biothiols play a crucial role in various physiological processes, including signal transmission, detoxification, and gene expression. Abnormal levels of biothiols are often associated with numerous diseases such as neurological disorders and liver damage, making them essential factors in clinical diagnoses. Consequently, there is a growing interest in developing new sensors to detect these biothiols. However, many existing methods have limitations such as time-consuming processes and the need for professional skills, restricting their practical application in resource-limited environments. In this study, a novel nanozyme-based colorimetric sensor array using Co3[Fe (CN)6]2 with a CoFe2O4 magnetic core derived from a Prussian blue analog is introduced for the detection of five different biothiols. The synthesized nanozymes underwent thorough characterization using FT-IR, XRD, FE-SEM, EDS, and VSM, confirming their suitability for catalytic reactions. The research focuses on the oxidation of chromogenic tracer agents (TMB, 4-AAP, ABTS, OPD, and DPD) in the presence of H2O2 as the oxidizing agent and CoFeO@CoFeCN nanozyme as the OH• radical activator. The results revealed distinct color changes (green, blue, magenta, dark pink, and dark yellow) for the nanozyme, and in the presence of each target biothiol, color intensity decreased. Digital images were recorded using a smartphone and analyzed with relevant software. Operating variables such as response time, pH, nanozyme concentration, chromogenic tracer concentration, and oxidizing agent concentration were optimized to achieve the best results. The optimum conditions included pH values ranging from 3 to 5, nanozyme concentrations between 0.2 and 1.4 % w/v by weight, chromogenic tracer concentrations from 0.6 to 1.2 mM, and oxidizing agent concentrations between 0.05% and 0.8% v/v. The sensor exhibited excellent performance with suitable (0.1 to 1000 µm) ranges and detection limits for biological thiols, including L-glutathione, L-cysteine, dithiothreitol, acetylcysteine, and cysteamine, on CoFeO@CoFeCN nanozyme. The calculated detection limit values were 1.5, 1.5, 0.15, 0.29, and 0.03 µM, respectively. The proposed sensor demonstrated good selectivity and reproducibility, performing well in the detection of target species in human blood plasma and serum samples.
