سيده مائده سمائي

The purpose of this project is to prepare composite pastes consisting of bioactive glass, polymer solutions containing gelatin and sodium alginate, cross-linking agent (GPTMS) and to investigate the biological and mechanical properties of the prepared composites. In this project, the type and amount of polymer solution was investigated as a variable. Injectable pastes are minimally invasive, low cost, and suitable scaffolds for encapsulating and transporting drugs. Bioactive glasses have the ability to stimulate biological response at the interface of materials. Gelatin polymer has biocompatibility and biodegradability properties. Sodium alginate polymer is highly hydrophilic and has the ability to form a viscous liquid. In the current study, bioactive glass (BG) 64SiO2.31CaO.5P2O5 was synthesized by acid and base sol-gel method and the characteristics of the synthesized powder were determined by XRD, FTIR analysis. Bioactive glass was mixed with different concentrations of polymer solutions consisting of gelatin (3 w/v%) and sodium alginate (3 w/v% and 6 w/v%) with a powder-to-liquid ratio of 0.25 (P/L = 0.25) and their injectability characteristics, mechanical properties, washing resistance, bioactivity and pH changes in (Simulated body fluid) SBF over time were investigated using XRD, FTIR, SEM and EDXA techniques was investigated, Finally, the toxicity level of nanocomposite pastes was eva‎luated by MTT eva‎luation. The results of XRD analysis confirmed the amorphous nature of the bioactive glass. The prepared paste of bioactive glass and gelatin (BG-Gel 3%) in the simulated body solution does not have washing resistance and a small amount of washing resistance of this paste was expressed as 0%. By increasing the concentration of sodium alginate polymer solution, the resistance to leaching of pastes increased, so that the paste prepared from bioactive glass and alginate (BG-Alg6%) has the highest resistance to leaching at about 98%. After immersing the pastes in SBF solution, a layer of calcite was formed on the surface of the pastes, which indicates the bioactivity of the nanocomposite pastes. The FTIR pattern of pastes is similar to the FTIR pattern of bioactive glass. SEM images showed flower-like morphologies indicating hydroxyapatite on the surface of the pastes after 14 days of immersion in SBF solution. BG-Gel3% and BG-Gel-Alg3% pastes did not have consistency during injection, however, all prepared pastes were completely removed from the syringe. The mechanical properties of composite pastes increased with increasing sodium alginate concentration. BG-Alg6% paste has a compressive strength of 2.390 MPa. MTT results showed that BG-Gel3% and BG-Alg6% nanocomposites are not cytotoxic. In general, composites prepared with bioactive glass, gelatin solution, sodium alginate solution and GPTMS can be used as bone substitutes.

شيشه زيست فعال , كامپوزيت , ژلاتين , سديم آلژينات , gptms

bioactive glass , composite , gelatin , sodium alginate , gptms

seyedeh maedeh samaie

dr. maryam tajAbadi- dr. hamidreza rezaie