ارزو مصطفوي

In this thesis, a green carrier of metal organic framework coated with natural polymers chitosan and carboxymethyl cellulose was utilized for the controlled release of the antibiotic drug levofloxacin. The performance of these two was compared. The structural properties of the prepared samples were investigated using various techniques such as X-ray diffraction, Fourier-transform infrared spectroscopy, and scanning electron microscopy. The release of the drug in an in vitro simulated environment was carried out using visible-ultra violet spectroscopy. The results indicated that the synthesized carriers have exceptional capability in drug loading (89.53%) and slow and controlled release in non-target sites, with appropriate release at the target sites. Specifically, for the NH2-UiO-66 coated with chitosan, a release amount of 69% at pH = 7.4 and 86% at pH = 5.5 was achieved. Similarly, for the NH2-UiO-66 coated with carboxymethyl cellulose, a release amount of 18% at pH = 7.4 and 78% at pH = 5.5 was attained. After eva‎luating the antibacterial assessment by determining the zone inhibition, the free drug exhibited higher antibacterial properties compared to drug loaded carriers, indicating their controlled release over a period of 24 hours. Additionally, the MIC and MBC concentration for drug loaded NH2-UiO-66 coated with chitosan and carboxymethyl cellulose, determined to be 0.25, 0.5 and 0.5, 4 mg/ml for E. Coli bacteria, respectively, which was lower than that for S. aureus bacteria. On the other hand, through cytotoxicity study, it was determined that after 48 hours, nanoparticles containing carboxymethyl cellulose at a concentration of 8 mg/ml showed lower cell viability (50.1%) compared to nanoparticles containing chitosan (61.11%). Therefore, the synthesized nanoparticles in this study can be considered as a new drug delivery system for the release of levofloxacin with promising antibacterial potential.

NH2-UiO-66 , كيتوسان , كربوكسي متيل سلولز , لووفلوكساسين , انتقال دارو , پاسخ به محرك pH

NH2-UiO-66 , Chitosan , Carboxymethyl cellulose , levofloxacin , drug delivery , pH-stimuli responsive

Arezoo Mostafavi

Dr. Mansoor Anbia