چكيده به لاتين
Today, nanotechnology plays a significant role in improving and controlling drug delivery and reducing the side effects of drugs compared to conventional treatment methods. Therefore, designing a biocompatible nanocarrier with high half-life, bioavailability and effectiveness in order to release medicinal compounds to the skin seems necessary. Local drug delivery is a safe method compared to other drug delivery routes, but the skin has different layers that make it difficult for the drug to penetrate it. In this regard, in this research, nanocomposite hydrogel based on carboxymethylcellulose-starch-Ag-GQD as a carrier for better penetration. and the controlled release and reduction of side effects of doxycycline drug were successfully synthesized for the treatment of bacterial infections and acne by emulsion method. The physical and chemical characteristics of the resulting nanocomposite were determined using analyzes such as FTIR, XRD, DLS, Zeta, FESEM, PL, UV-vis. Then doxycycline drug was loaded on the above nanocomposite and the capacity and efficiency of drug loading in the nanocomposite hydrogel was checked, and the results showed an increase in the capacity and efficiency of drug loading in the synthesized nanocomposite hydrogel. Also, by adding quantum graphene dots coated with silver nanoparticles, a synergistic effect was observed for the drug-containing nanocomposite in treating bacterial infections and increasing the antibacterial properties of the drug delivery system. The results of FTIR, XRD, FESEM analyzes confirmed the formation of carboxymethylcellulose-starch-Ag-GQD nanocomposite containing doxycycline. The surface charge values of the nanocomposite hydrogel and the hydrodynamic diameter range of the nanosystem were obtained by Zeta and DLS analysis as -48 mV and 233 nm, respectively, which indicated the good stability of the nanocarriers and the favorable particle size distribution of the nanocomposites for pharmaceutical applications. The release rate of doxycycline from the nanocomposite was investigated using the dialysis bag method and with consecutive sampling after 6, 8, 24, 48 hours, using UV spectrophotometry and the doxycycline standard calibration curve. The results of the drug release from the nanocomposite hydrogel showed a stable and controlled pH-sensitive release in the acidic environment of the skin cell, with a passive targeting mechanism that leads to a reduction in the toxic effects of the drug for healthy cells. Disk diffusion test were performed to check the antibacterial effect of nanocomposite and to check the synergistic effect of silver nanoparticles and nanocomposite containing doxycycline.
