ارشيا قاضيان

Considering the penicillin G-induced drug poisoning and also the adverse effects caused by the widespread use of antibiotics, which leads to the spread of antibiotic resistance, the measurement of this antibiotic is important from a medical and judicial point of view. In the current research, an electrochemical aptasensor has been designed and fabricated based on nanocomposite resulting from triangular silver nanoplates and manganese trioxide nanocubes for accurate identification and detection of penicillin G. The proposed nanocomposite with unique advantages was synthesized easily and cost-effectively via two steps and used as the modifier on the surface of the glassy carbon electrode. The aptasensor was fabricated by attaching the aptamer as the bioreceptor for penicillin G on the nanocomposite immobilized on the glassy carbon electrode surface. By incubating the target molecule on the embedded sensing interface and the aptamer configuration change for trapping the penicillin G the electrochemical signal related to the ferro/ferrocyanide probe was changed. Thus, Penicillin G was measured in the linear concentration range of 1-2700 nanomol per liter with a detection limit of 330 picomol per liter based on the electrochemical impedance technique. It is measured in femtomol per liter. The analytical efficiency of the proposed aptasensor for rapid and selective determination of penicillin G in real samples of urine, saliva and cow milk was well eva‎luated as a non-invasive method.

نانوصفحات مثلثي نقره، نانومكعب دي منگنز تري اكسيد، پني¬سيلين جي، آپتاحسگر الكتروشيميايي، سنجش غيرتهاجمي.

Triangular silver nanoplates, Dimanganese trioxide nanocubes, penicillin G, Electrochemical aptasensor, Non-invasive assay.

Arshia Ghazian

Dr. Ali Ghaffarinejad