محمد بريهي

In this thesis, two research projects were conducted. In the first study, a composite ratiometric sensor based on the dual metal-organic frameworks Amino-MIL-53@MO/ZIF-8 and Rhodamine B was used for creatinine detection. Metal-organic frameworks (MOFs) served as the fluorescent species for creatinine measurement, while Rhodamine B acted as a reference component to minimize interferences in the sensing system. This sensor exhibited high selectivity and sensitivity in detecting creatinine. As creatinine concentration increased in the ratiometric probe, the fluorescence signal of the MOFs at a wavelength of 425 nm decreased, while the Rhodamine B signal at 580 nm remained constant. Key parameters such as pH, the volume ratio of MOFs to Rhodamine B, and interaction time were optimized using a one-variable-at-a-time method. The calibration curve under optimal conditions was linear for creatinine in the range of 0.35–11.1 µM. The calculated detection limit and quantification limit were 0.18 and 0.54 nM, respectively. The repeatability and reproducibility were found to be 0.67% and 0.84%, respectively. The satisfactory results obtained from creatinine measurement in real samples indicated the practical applicability of this sensor. In the second study, a smart nanocarrier for oral drug delivery was developed to reduce side effects and enhance effectiveness. To increase drug loading capacity, NMOF-74 with magnesium as the central metal was used. Due to its small particle size and large surface area, NMOF-74 provided numerous active sites for drug loading, achieving a loading efficiency of 97.9%. Thus, NMOF-74 demonstrated a high drug-loading capacity. The central composite design based on response surface methodology was applied to optimize drug loading conditions and eva‎luate key parameters: time = 25 minutes, NMOF-74 dose = 15 mg, and 5-fluorouracil concentration = 200 mg/L. Subsequently, 5-FLU-NMOF-74 was coated with polyacrylic acid (PAA). Based on the release behavior of PAA-5-FLU-NMOF-74 in different pH environments, it can be concluded that the nanocarrier exhibited smart release in the intestine compared to the stomach. Kinetic studies revealed that the drug release mechanism of 5-fluorouracil from the PAA-5-FLU-NMOF-74 nanocarrier followed Fickian diffusion. To eva‎luate the efficacy, the toxicity of PAA-5-FLU-NMOF-74 was studied in the presence of MCF-7 and HEK293T cell lines. PAA-5-FLU-NMOF-74 did not show significant toxicity at all concentrations. Moreover, PAA-5-FLU-NMOF-74 was more toxic to MCF-7 cells than the free drug at all concentrations.

كراتينين , 5-فلوراراسيل , كامپوزيت نانوذرات-چارچوب هاي فلز آلي , حسگر/پروب نسبت سنجي , رودامين-ب , دارورساني هوشمند , پلي آكريليك اسيد

Creatinine , 5-fluorouracil , NPs@MOFs Composite , ratiometric sensor , Rhodamine b , Smart drug delivery , Polyacrylic acid

mohammad bereyhi

Rouholah Zare Dorabei