فاطمه چگيني

In this study, a silica-based hybrid nanocarrier was designed, synthesized, and eva‎luated to improve the delivery of amoxiclav to methicillin-resistant Staphylococcus aureus (MRSA). Silica nanoparticles were prepared by sol-gel method and coated with a polyvinyl alcohol (PVA)-based polymer functionalized with guanidinium groups to provide biological functionality and effective targeting. The functionalization process was performed in two steps: first, chemical coupling of 3-aminopropyltrimethoxysilane (APTMS) to PVA chains, and then guanidinium using cyanamide to induce antibiotic-independent antibacterial properties in the final pharmaceutical formulation. In addition to amoxicillin, clavulanic acid was also used as a specific inhibitor of β-lactamase enzyme to simultaneously target bacterial enzymatic resistance. The final nanocomposite was characterized and confirmed by FTIR, FESEM, BET, XRD, Zeta and DLS analyses. The results of these analyses indicated the formation of a stable structure with a uniform particle size in the range of 60–70 nm. The drug loading efficiency was estimated to be about 90%, which indicates the high ability of the nanocarrier in drug adsorption and stabilization. In vitro antibacterial activity tests showed that the designed nanocarrier, compared to the free form of the drug, has a much stronger inhibitory effect on resistant MRSA strains. The obtained results indicate the high potential of this nanosystem in overcoming bacterial resistance, through the simultaneous delivery of antibiotic and enzyme inhibitor, along with the enhancement of antibacterial effects due to the presence of guanidine groups in the carrier structure.

دارورساني , آنتي ميكروبيال , ميسل , نانو حامل , نانو ذره سيليكا متخلخل , آموكسي كلاو , رهايش كنترل شده

Drug delivery , Antimicrobia , Micelles , Nanocarriers , Porous silica nanoparticles , Amoxiclav , Controlled release

Fateme Chegini

Dr. Ali Maleki