طه جعفري

Breast cancer is one of the most common an‎d deadly cancers among women in the world, an‎d early detection plays a key role in increasing the chances of treatment an‎d reducing mortality. In recent years, the development of electrochemical biosensors as rapid, accurate, an‎d low-cost tools for identifying cancer-related biomarkers has attracted much attention. The aim of this study was to develop an electrochemical biosensor based on gold nanoparticles (AuNPs) coated on a printed electrode (SPE) for the detection of breast cancer in lymphatic fluid. In this study, first, printed electrodes were coated with a uniform layer of gold nanoparticles using DC Magnetron Sputtering to improve conductivity, stability, an‎d charge transfer on the electrode surface. Then, the surface modification process was performed using mercaptopropionic acid an‎d EDC/NHS activation to enable covalent attachment of a specific antibody against HER2 receptors. After immobilization of the antibody, SKBR3 (HER2⁺) cells as a breast cancer model were immobilized on the electrode surface an‎d the electrochemical response was measured using cyclic voltammetry (CV), square wave (SWV) an‎d electrochemical impedance spectroscopy (EIS) methods using a Palm device. The results showed that the presence of gold nanoparticles resulted in a significant increase in electron transfer an‎d a decrease in charge resistance (Rct). The developed biosensor detects SKBR3 cancer cells in a range of 100 to 400 cells. The limit of detection (LOD) of the sensor was about 5.6 (EIS) an‎d 38.9 (SWV) cells, respectively, which indicates high specificity an‎d selec‎tivity compared to similar sensors. The overall conclusion of the research shows that the combination of gold nanoparticles with modified printed electrodes provides a suitable platform for the development of portable, accurate, an‎d cost-effective biosensors. This possibility could be used as a step towards early an‎d non-invasive detection of breast cancer in lymph nodes.

زيست‌حسگرهاي الكتروشيميايي , سرطان سينه , غده هاي لنفاوي , پوشش طلا , سلول‌هاي SKBR3

Electrochemical biosensors , breast cancer , , lymph nodes , gold coating , SKBR3 cells

Taha Jafari

Dr. Seyed Morteza Naghib