Design of Nanocomposites Based on Metal–Organic Frameworks Modified with Carbon Materials an‎d Metallic Nanoparticles for Application in a SARS CoV 2 Diagnostic Nano-genosensor

5/4/2027 12:00:00 AM

This study is devoted to the development an‎d eva‎luation of two novel nanogenosensors for the sensitive an‎d selec‎tive detection of genomic sequences of the SARS CoV 2 virus. The first nanogenosensor was designed for the detection of the RdRp gene of SARS CoV 2, based on a fluorine doped tin oxide (FTO) modified electrode with a nanocomposite consisting of sul-fur doped reduced graphene oxide (S rGO), dendritic palladium nanostructures (den PdNS), an‎d a cerium based metal–organic framework (Ce MOF). The fabricated nanogenosensor exhibited a remarkable limit of detection (LOD) of 0.2 fM, an appropriate sensitivity of 67.10 μA cm-2, an‎d a wide linear range from 10 fM to 10 μM for the concentration of the RdRp gene of SARS CoV 2. The results obtained from the performance of the nanogenosensor in human saliva samples showed a recovery between 93 an‎d 104 ⁒. The second nanogenosensor was designed for the detection of the N gene based on an FTO elec-trode modified with a nanocomposite composed of nitrogen doped reduced graphene oxide (N rGO), dendritic gold nanostructures (den AuNS), an‎d a zirconium based metal–organic framework, MIP 202(Zr). The developed nanogenosensor platform for the detection of the SARS CoV 2 N gene was eva‎luated within a concentration range from 10 fM to 10 μM an‎d demonstrated a very high sensitivity of 24.18 μA cm-2 an‎d a limit of detection of 0.67 fM. Fur-thermore, the performance of the nanogenosensor in human saliva samples resulted in a recovery between 104 an‎d 108 ⁒. Both designed nanogenosensors showed remarkable sensitivity, selec‎tivity, an‎d stability, which was achieved through the incorporation of engineered nanostructures in the electrode platform. The selec‎tivity, reproducibility, repeatability, an‎d long term stability of both biosensors were comprehensively eva‎luated, an‎d the results confirmed their reliability for practical diagnostic ap-plications. In addition, validation studies using clinical samples, including human saliva, con-firmed the efficiency of these biosensors in detecting SARS CoV 2 at very low concentrations. The results of this study demonstrate the potential of nanogenosensor technology as a rapid, non invasive, an‎d highly sensitive tool for the detection of COVID 19. These advancements play an important role in the development of efficient an‎d accessible biosensing systems for pan‎demic management an‎d the detection of emerging viruses.

زيست‌حسگر الكتروشيميايي , سارس-كوو-2 , كوويد-19 , نانو مواد كربني , نانو ساختار دندريتي فلزي , چارچوب¬ فلز-آلي

Electrochemical biosensor , SARS-CoV-2 , COVID-19 , Carbon-based nanomaterials , Metallic dendritic nanostructures , Metal-organic framework

Niusha Shishegari

Azadeh Tadjarodi, Eskandar Omidinia