چكيده به لاتين
Abstract:
Molecular imprinting is one of the new methods for selective separation of different compounds. Due to the sensitivity of biomolecules to changes in operational conditions like high temperature or using different solvents, this method has attracted much attention in recent decades. Because of importance of improving separation conditions or making new methods like molecular imprinting commercial, also because of few experimental researches on molecular imprinting of amino acids, lack of experimental data on molecular imprinting of Lysine and comparison of obtained results with existing results in bulk imprinting of Lysine, in this survey separation of L- Lysine was conducted. Different parameters such as functional monomer and total functional monomer, crosslinking percentage, pH of absorption step and different solvents in extraction step of the template were examined for optimization. According to conducted experiments and mentioned optimizations, the optimized hydrogel was the sample with the crosslinking percentage of 15%, a total functional monomer of 6.25 and best pH for absorption step was 7.8.
In absorption step, 98% of recovery was obtained which is almost a good result. But in releasing step, results had some defects which should be investigated more. These deficiencies could be because of hydrogel synthesis process and the polymer network or using an inappropriate solvent in the extraction of Lysine form bonding sites. Solving these problems in extraction step could be a good point for starting new research in this field.
Keywords: Molecular Imprinting, Hydrogel, L-Lysine, Chinard Colorimetery Analysis, Amino Acids.
