چكيده به لاتين
After a myocardial infarction, known as a heart attack, blood flow and oxygenation are impaired on the heart muscle that causes the death of the heart cells in that area. The damaged heart muscle loses its ability to contract, and due to the replacement of heart-damaged muscles with fibrous scar, structural and functional changes occur in the heart muscle. This scar can not contract regularly and is not a good navigator for electrical signals. By developing the proper conditions for the combination of cells and three-dimensional scaffolds, heart tissue engineering allows for a mechanical protective structure for heart cells, as well as the presence of heart cells to repair damaged tissue. This three-dimensional structure known as cardiac patch is grafted to the infarcted area and improves cardiac efficiency. In this study, random and aligned polyurethane / reduced graphene oxide composite nanofibrous scaffolds were electrospun as scaffolds for cardiac patches and mechanical, chemical and structural properties of scaffolds were investigated by scanning electron microscopy (SEM), water contact angle, attenuated total reflectance Fourier transform infrared spectroscopy and tensile measurements. Then, satellite cells of mouse were cultured on scaffolds and the effect of these properties on the growth, morphology, proliferation, differentiation and expression of cell genes was investigated using Real-Time PCR method. Results indicated that the presence of nanoparticle improved the mechanical properties of scaffolds and the orientation of fibers caused to a better resemble of structural and mechanical properties of cardiac tissue by showing anisotropic wetting characteristics. Overall, the improvement of these properties and their close proximity to the properties of the normal ECM of the heart, improved the growth and differentiation of mouse satellite cells into cardiac prognostic cells.
