چكيده به لاتين
Plasma Medicine, as an evolving branch of modern medicine, has provided novel ways to treat many diseases. On the other hand, emerging medical fields require reliable stem cell resources for biomedical research. Nowadays, Mesenchymal stem cells are widely used in transplantation and treatment of various lesions and abnormalities of the nervous, cardiac, skeletal, and so on. Adipose derived stem cells are Mesenchymal stem cells derived from abdominal adipose tissue and can be cultured in cell culture flasks in vitro. Studying the behavior of these cells against cold plasma radiation can be of great help in future research in the field of wound healing and a variety of diseases. In this study, Adipose derived stem cells isolated and cultured. After the fourth passage, these cells are irradiated with helium plasma jet. Then, using flow cytometry technique, surface markers, cell viability, cell count, apoptosis, cell cycle and intracellular reactive oxygen species were investigated. One of the important results of these experiments is the effect of irradiated time on cell death. As the plasma dose increases, the cells begin to apoptotic and necrosis. At very low irradiation times, early apoptosis was observed due to oxidative stress, and only at an optimal time cell death decrease and proliferation increase. By examining the cell cycle, it was found that cell death depends on the cell position in the cell cycle and by evaluating the expression of mesenchymal markers, a slight effect of plasma irradiation on cell differentiation is observed
