چكيده به لاتين
Nowadays, application of validated and advanced analytical techniques to determine different compounds is a critical issue in food industry. In the present study, 4 researches have been done for the development and validation of innovative liquid phase microextraction technique followed by HPLC and GC-MS to extract trace amount of bioactive compounds and pllutant in food samples. The aim of two researches is the development and validation of sensitive and accurate dispersive liquid-liquid microextraction coupled with HPLC to determine the amount of vitamin D3 in fortified cereal and dairy products was determined. Resultes showed that the proposed method has high capable to determine vitamin D3 in two high-consumption food samples in Iran. According to critical role of vitamin D3 in human health, the aim of these projects was to develop of DLLME method in food industry to determine vitamin D3. In the third research, with the aim of development, validation and innovation of LPME in food science and increasing the selectivity, sensivity and extraction efficiency, µDLLME and electromembrane extraction have been joined to improve dynamiclinear range, sensitivity and selectivity. The application of new proposed technique has been investigated with determination the amount of biogenic amines in canned fish. The effective parameters have been optimized using central composite design. Limit of detection and limit of quantitation of proposed technique were obtained in the range 0.03-0.1 ng g-1 and 0.09-0.36 ng g-1. In the forth research, for the firs time, microchip system connected to HPLC in online mode has been introduced and validated. Capability of the proposed method to detect the amounts of heterocyclic aromatic amines was investigated in grilled meat samples. The important factors in extraction process of target analyte in grilled meat have been optimized. Increasing of accuracy, sensitivity and selectivity are the advantage of proposed method. One of the most important advantages of the offered method is the decreasing of the volume of organic solvent in extraction process, significantly. High recovery (<0.95), high enrichment factor, repeatability (2.9- 5.6%) are the other advantage of proposed method.
