چكيده به لاتين
MS is a progressive inflammatory disease (neurodegenerative) and autoimmune in which the myelin sheath is responsible for transmitting nerve damage, and the rate of transmission of neural messages slows down and leads to motor disorders. Iran ranks first in the Middle East. Early diagnosis of this disease can prevent recurrence of the disease and side effects. Existing emarush is very laborious and expensive, and due to its similarity to Parkinson's and Alzheimer's disease, sometimes the exact diagnosis of this disease is not made. Biomarker, biochemistry is used as an indicator in the diagnosis and treatment of diseases. MicroRNAs are small, non-coding RNAs that are abnormally expressed in many diseases and have been identified as predictive biomarkers for early detection of diseases. According to studies, one of the best diagnostic biomarkers of this disease is miR-155. In this project, a high-sensitivity, low-cost, non-label-sensitive electrochemical nano biosensor is used to identify miR-155 by the formation of single-walled carbon nanotubes (SWCNT) nanocomposites to increase the active surface area, accelerate the kinetic acceleration of electron transfer, reduce overvoltage and increase electrical conductivity. Improving the stabilization of biomaterials as the interface between the biological receptor and the electrode surface and increasing the electrical conductivity by electrochemical method for the first time on the surface of the graphite electrode (GS) should be synthesized.
The amine-containing complementary strand was attached as a probe for hybridization with the target molecule miR-155 as a complementary sequence, and a significant change was observed in the probe amperometry answer in Fe(CN)63-/4- as a signal generating element in the solution. . The Detection limit is 10 aM and dynamic range is 10aM-1µM and probe detection range, respectively, indicate the high performance of this biosensor
