حديث ابوحيدري پوركرماني

Treating wounds is expensive and challenging. Most of the existing wound dressings are not effective and suffer from limitations such as poor antimicrobial activity, toxicity, inability to provide adequate wound moisture, and poor mechanical performance. Using inappropriate wound dressings can lead to a delay in wound healing. Nanoscaffolds have attracted much attention due to their features such as the ability to deliver bioactive agents, high surface-to-volume ratio, improved mechanical properties, and extracellular scaffold (ECM) mimicry. Nanofibrous materials can be further encapsulated or loaded with metal-based nanoparticles to enhance their therapeutic outcomes in wound healing applications. In this research, a wound dressing consisting of silk, polyethylene oxide, honey and silver nanoparticles was made using the electrospinning method. FTIR spectrum showed that honey was successfully loaded into SF nanofibers. Nanofibers show random orientation and their diameter increases with increasing honey content and decreases with the addition of silver nanoparticles (the diameter of silk fibroin nanofibers, polyethylene oxide, 10% honey and silver nanoparticles (SF-P-H10-Ag) and silk fibroin, polyethylene oxide, 15% honey and silver nanoparticles (SF-P-H15-Ag) respectively 450 ± 150 nm and 650 ± 150 nm). In addition, water absorption, biodegradability and mechanical properties were also investigated. The amount of water absorption of the SF-P-H10-Ag sample is 12% more than that of the SF-P-H15-Ag sample and the water vapor transfer rate of the SF-P-H10-Ag sample is 15% more than that of SF-P-H15-Ag is increasing the amount of honey, the rate of degradation of nanofibers increased (the rate of degradation of SF-P-H10-Ag and SF-P-H15-Ag nanofibers after 14 days was 47.14 ± 15.3% and 35.46 ± 14.97%, respectively). Antimicrobial eva‎luation by disk diffusion method shows that nanocomposite scaffolds have good antimicrobial activity and the halo diameter of SF-P-H10-Ag and SF-P-H15-Ag nanofibers against S. aureus is 16 mm and 19 mm, and against P. aeruginosa is 15 mm and 18 mm, respectively. Also, the survival percentage of cells in SF-P-H10-Ag and SF-P-H15-Ag nanofibers after five days is 82.44% and 79.24%, respectively. Our findings suggest that composite scaffolds prepared by combining SF, honey and silver nanoparticles can be a promising candidate for wound dressing.

فيبروئين ابريشم , ضدميكروبي , الكتروريسي , نانوذرات نقره , زخم پوش‌ها , عسل

Silk fibroin , Antimicrobial , Electrospinning , Silver nanoparticles , Wound dressings , honey

Hadis Aboheidari pourkermani

Dr. Tajabadi - Dr. ghanbari