اميررضا جباري

Bioactive glasses have ideal bioactive and bone-bonding properties. If these glasses synthesize in a spherical shape, they can be facilely used in many applications. Pore formation in their structure leads to a sharp increase in surface area and faster dissolution. After the dissolution, the glasses gradually release ions and species with biological effects in the targeted tissue. Manganese with osteogenic and antibacterial effects and cobalt with angiogenetic properties are among the ions with biological effects. This research is about the effects of the addition of these two elements to the bioactive glass individually and simultaneously to achieve a multifunctional glass. In this study, firstly, mesoporous bioactive glass nanospheres with mol% composition of SiO2 80, CaO 16, P2O5 4 are synthesized, then the effects of the addition of manganese and cobalt on the glass properties, simultaneously and individually, are investigated. The synthesis was conducted with the modified Stöber method (using a basic catalyst). Cetyltrimethylammonium bromide (CTAB) is used as a templating agent for pore formation. At first, the synthesis began with water and ethanol solvents, and then for modifying particle size methanol is substituted for ethanol. The synthesis method is modified regarding the washing procedure and the order of addition of the reagents. Samples are prepared with manganese (2 mol%), cobalt (2 mol%), and manganese and cobalt (1 mol% each) addition, and then are characterized. The results showed an amorphous and spherical shape of the glasses. With changing the solvent to methanol, particle size showed a significant decrease from almost 194 nm to 100 nm. The samples containing Mn and Co also have a spherical morphology and using the N2 adsorption-desorption analysis presence of mesopores is identified. Based on the results of the X-ray diffractometer, the amorphous structure after the addition of Mn and Co is preserved. The presence of these added elements in the synthesized spheres is identified using X-ray fluorescence spectroscopy. The addition of Mn and Co to samples increased the specific surface area of the initial bioactive glass (800 m2/g) to 908 and 857 m2/g, respectively. The specific surface area of the sample with both oxides (820 m2/g) was lower than the samples with just one of the oxides. Based on the results of bioactivity eva‎luation using body-simulated fluid, hydroxyapatite formation on the samples containing Mn/Co was lower than the sample with no addition. The hydroxyapatite phase did not appear in the X-ray diffraction pattern of the sample with both elements after 14 days. Based on the cytotoxicity assay, only the glass with 1 mol% of manganese and cobalt showed no cytotoxic effect.

شيشه زيست‌فعال , نانوكرات , مزومتخلخل , منگنز , كبالت

bioactive glass , nanospheres , mesoporous , manganese , cobalt

Amirreza Jabbari

Bijan Eftekhari Yekta - Hamidreza Rezaie