فرناز قرباني

Tissue engineering, along with medical science, deals with reviving and restoring damaged tissues and organs. Scaffolds play a crucial role in tissue repair and regeneration. Choosing the type and material of the scaffold is very important because it eventually replaces the damaged tissue. The development of bioactive ceramic composite scaffold materials with improved mechanical properties has been a topic of interest in bone tissue engineering. In the present study, Hydroxyapatite-gelatin scaffolds were fabricated by using digital light processing (DLP) based additive manufacturing in an indirect method with gradient porosity. Here, the percentage of gelatin (5 and 7% by weight) and thermal cycle-in order to remove the photopolymer resin and sintering of hydroxyapatite - is considered as a variable. Scanning Electron Microscope (SEM), X-Ray Diffraction (XRD), and X-ray Energy Diffraction Spectroscopy (EDS) are used to observe the morphology, microstructure, and size of the pores and compositions of the scaffolds. The porosity of the scaffolds was estimated between 60 and 80%.The eva‎luation of mechanical properties was done by Compression test and the results showed that the compressive strength of the samples is in the range of 1.15-64 MPa which was completed within the compressive strength range of cancellous bone (.2-4 MPa). Moreover, cell studies were performed to confirm the biocompatibility, lack of toxicity, and differentiation into bone cells. In the following, the cell adhesion and cell viability on the optimal scaffold in vitro conditions were performed using MG63 cells. The result of Alizarin red staining also showed that the mesenchymal stem cells have differentiated well into the bone line.

داربست استخواني ، هيدروكسي آپاتيت ، پرينتر سه بعدي،زيست سازگاري

Bone tissue engineering, scaffold, hydroxyapatite, 3D printer, biocompatibility

Farnaz Ghorbani

Dr. Alireza Khavandi