فاطمه وطن دوست

Graphene oxide is considered as an ideal candidate for targeted drug delivery systems, because it is chemically stable, biocompatible, has a large surface area and low cost. On the other hand, graphene oxide sheets tend to aggregate due to van der Waals bonds and large specific surface area, thus reducing drug loading ability and water solubility. The strategy of functionalizing graphene oxide with polymer and magnetic nanoparticles is not only a useful approach to dissolve graphene oxide in water, but also improves stability, reduces toxicity and develops drug delivery hybrids. The purpose of this research is to design a drug release system with magnetic graphene oxide combined with chitosan/glycerophosphate hydrogel with the aim of releasing curcumin drug for cancer treatment, so that the weaknesses of the traditional drug release system can be covered, and benefited from the characteristics of graphene oxide, magnetic property and hydrogel structure of its substrate in this drug delivery system. In this way, the desired drug is transferred to the target tissue with minimal side effects. After designing the final chitosan/glycerophosphate/magnetic graphene oxide hydrogel along with curcumin drug as a smart pharmaceutical system, XRD, FTIR, VSM, SEM, MTT analyzes were performed to check the performance of this system, And the drug release profile of curcumin from this system was investigated. According to the results obtained from FTIR analysis, a physical hydrogel was obtained without creating a chemical bond. The morphology of the hydrogel surface using SEM showed that increasing the concentration of magnetic graphene oxide increased the porosity from 74.3% to 83.1% and the size of the pores increased from 40.4 ± 13.8% to 52.8 ± 32.8%. Also, the inclusion of magnetic graphene oxide improves the swelling percentage from 6 ± 0.6% to 12 ± 1.1% in one hour and reduces the degradation rate from 39 ± 2.8% to 17 ± 1.2% in 7 days. Also, MTT analysis determined that magnetic graphene oxide nanoparticles in the right concentration not only do not show toxicity, but increase cell viability by 2.45% compared to chitosan hydrogel without nanoparticles. In the eva‎luation of curcumin drug release, it was also observed that the drug is released from the hybrid system of chitosan with magnetic graphene oxide nanowires more slowly and more controlled than chitosan hydrogel alone and nanoparticles without hydrogel substrate, So that within 40 days, 34.29% of the drug was released from the system. Therefore, it can be concluded that adding magnetic graphene oxide nanoparticles to the structure improves the properties of the system without changing the porous microstructure.

اكسيد گرافن، خاصيت مغناطيسي، هيدروژل حساس به دما، كيتوسان، رهايش دارو، سرطان

Graphene oxide, magnetite, Temperature sensitive hydrogel, chitosan, drug release, cancer

Fatemeh Vatandoost

Dr. Maryam Tajabadi - Dr. Maryam Salehi