چكيده به لاتين
Diabetes, a prevalent metabolic disorder worldwide, poses a significant public health concern. The monitoring of biomarkers in human sweat represents a promising avenue for disease identification. Among the various biomarkers that indicative of diabetes found in human sweat, glucose, L-Tryptophan, uric acid, and acetone stand out. To address this challenge, we present a novel approach in our current research: the development of a nanozyme-based colorimetric sensor array. This array is constructed using a Ce/Fe Metal Organic Framework (MOF) doped with copper and nickel, further modified with silver nanoparticles. Comprehensive characterization of the synthesized nanozymes was conducted using FT-IR, XRD, FE-SEM, TEM, EDS, and ICP-OES methods, confirming their suitability for catalytic reactions. This study focuses on the oxidation of chromogenic tracer agents, namely 3,3′,5,5′-Tetramethylbenzidine (TMB), Ortho Phenylene Diamine (OPD), 2,2′-azino bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and 4-Amino antipyrine (4-AAP), with hydrogen peroxide as the oxidizing agent, in the presence of Ag/Cu/Fe-Ce-UiO-66-Fum and Ni/Fe-Ce-UiO-66-Fum nanozymes, generating distinct colors. When analytes are introduced, these colors fade, and the changes are recorded using a smartphone. Optimization of operational variables, including response time, pH (4.3 to 6.4), nanozyme concentration (0.27% to 0.5% w/v), chromogenic tracer agent concentration (0.2 to 1 mM), and oxidizing agent concentration (0.1% v/v), yielded the best results. This method exhibits a wide linear range (20 to 1000 µM) and low detection limits (5.73 µM for uric acid, 5.90 µM for L-Tryptophan, 5.85 µM for acetone, and 5.65 µM for glucose). Furthermore, it demonstrates remarkable selectivity, reproducibility, and performance when applied to human sweat samples. Our proposed colorimetric assay offers substantial promise for the detection of diabetes biomarkers and health monitoring via point-of-care systems, thereby advancing the field of human health diagnostics.
