مريم قياسي

In this Thesis, the design and fabrication of an electrochemical aptasensor based on nanomaterials obtained from waste matterials have been investigated to identify and detect metronidazole as a harmful drug in the environment. In this research, relying on the principles of green chemistry, carbon dots Efficiency was prepared from egg shell membrane waste and fixed as a modifier on the surface of the glassy carbon electrode as a Working electrode . Then, Silver nanocubes were used as a suitable binder for a better and more stable surface of the amino aptamer bioreceptor on the surface of the glassy carbon electrode. By examining the changes in the steric hindrance in each step of modifying the electrode surface and tracking the changes in the electrochemical signal related to the species in 0.1M KCl solution in the presence of Fe(CN)64-/3- redox prob manufactured by Aptasensor was approved for the electrochemical measurement of metronidazole. Finally the Detection limit of this assay was estimated to be 0.3 fM within the dynamic linear range of 1 fM to 901.4 nM. to eva‎luate the analytical efficiency of the designed Aptasensor, real, low-invasive samples of human saliva and urine were used, and good results were obtained. The proposed Aptasensor was Designed as a rapid measurement method in a short period, extremely sensitive, and optional for measuring the Concentration of metronidazole in saliva and urine samples.

آپتاحسگر الكتروشيميايي , ضايعات , غشاء پوسته تخم‌مرغ , ولتامتري , مترونيدازول , نانومكعب نقره

Electrochemical aptasensor , Waste , Egg shell membrane , Voltammetry , Metronidazole , Silver nanocube

Maryam Ghiasi

Dr. Ali Ghaffarinejad