سيده سارا حسيني

Bacterial contamination is a common problem and a serious threat to human health worldwide. Therefore, the development of multifunctional biomaterials with antibacterial properties and resistance to infection is an ongoing goal for biomedical applications. One of the recent ways to further expand the antimicrobial applications of metals is to use them as nanoparticles in polymer matrices. In this project, silk-based electrospun nanofibers and copper-based nanoparticles with different ratios of fuel to oxidant have been designed to make an antibacterial composite scaffold. The characteristics of the samples were eva‎luated by XRD, FESEM, EDX-MAP, FTIR, antibacterial and MTT tests. The XRD results confirmed the successful synthesis of copper-based nanoparticles by the solution combustion method, which has advantages such as the production of high-purity and cost-effective materials. FESEM images showed the average diameter for pure silk fibroin, nanofibers around 322 nm, and for nanocomposites with a ratio of 1, 1.5, and 2, respectively, nanofibers around 553 nm, 636 nm, 657 nm, and samples of pure silk fibroin nanofibers and Nanocomposites were produced by random orientation with smooth morphology (except for the sample with fuel-oxidant ratio equal to 1), uniform and without grains, under controlled parameters. FTIR results show the interaction between silk fibroin fibers and copper-based nanoparticles and the loading of copper-based nanoparticles inside the silk fibroin matrix. Based on the results of the antibacterial test, the diameter of the inhibition zone of the optimal sample for E.Coli was equal to 34 mm and S.aureus was equal to 36 mm. According to the MTT results, the survival of 375A cancer cells in the presence of the optimal sample decreased to 87.9% compared to the control group, which shows that this scaffold can be used as an anticancer agent. As a result, silk-based and copper-based nanoparticles electrospun nanofibers can work well as antibacterial composite scaffolds in various fields, including tissue engineering.

الكتروريسي , نانوذرات برپايه ي مس , فيبروئين ابريشم , خاصيت آنتي باكتريال , كامپوزيت

Electrospinning , Copper-based nanoparticles , Silk fibroin , Antibacterial property , composite

Sara Hosseini

Dr. Maryam Tajabadi