چكيده به لاتين
In this study, metal-organic frameworks (MOFs) ZIF-67 have been decorated with Ginkgo biloba plant extract and DNA to create a suitable and safe strategy for the simultaneous delivery of doxorubicin and sorafenib (DOX-SOR). Characterization using FT-IR, XRD, FESEM, and TEM confirmed the optimized structure of the modified MOFs, validating the successful synthesis and cubic structure. Drug loading revealed encapsulation of 59.7% and 60.2% for doxorubicin and sorafenib, respectively. HEK-293, HT-29, and MCF-7 cells treated with the samples at low and high concentrations (0.1 and 50 μg/mL) demonstrated excellent viability, indicating potential medical applications. Due to modifications in the carrier, drug release was prolonged and pH-sensitive, with the highest release occurring at pH 4.5. The internalization and drug delivery were examined using a 2D fluorescence microscope, confirming effective internalization in MCF-7 cells and demonstrating the efficacy of the drug delivery systems. Additionally, the mentioned natural biomaterials were used to decorate UiO-66-NH2, achieving drug encapsulation of 49.5% for doxorubicin and 31.2% for sorafenib. Viability studies on MCF-7, HT-29, and HEK-293 cell lines revealed excellent cell viability at both low and high concentrations. However, coating the nanocarrier with Ginkgo biloba leaf extract and E. coli DNA significantly reduced cell viability. Finally, for regenerative medicine development, hydrogel films of chitosan/collagen/polycaprolactone (CSCPs) were synthesized with different weight ratios of PCL (1:3, 1:6, and 1:9). Increasing PCL content resulted in higher elongation at break and tensile strength. Water contact angle tests showed increased hydrophobicity with higher PCL content. After 48 days, CSCP6 and CSCP9 hydrogel films exhibited slow degradation, losing less than 15% of their weight. All three types of hydrogel films demonstrated high biocompatibility (over 95% after three days), as confirmed by the MTT assay. The hemolysis rates of CSCP hydrogel films were less than 2%, making them safe for contact with blood. The absence of costly crosslinking agents and desirable characteristics for tissue engineering applications suggest that CSCP hydrogel films may be promising candidates for use in artificial tendons.
