چكيده به لاتين
The separation of micro and nano-sized particles and cells has always been regarded as a significant difficulty in the relevant scientific fields. The separation of cells, particularly blood cells, exemplifies these issues prominently. Pinched flow fractionation is a contemporary size-dependent technique for cellular separation. Given the system's significant potential, several approaches to enhance its performance have been investigated and developed in recent years. Given the existing constraints of this system, enhancing its performance via the integration of external factors is imperative. This study investigates the enhancement of separation performance for platelet cells, red blood cells, white blood cells, and circulating tumor cells in a blood medium by separately and simultaneously applying the forces of dielectrophoresis and magnetophoresis to pinched flow fractionation. To do this, the necessary conclusions were achieved by solving equations like the Navier-Stokes equation for fluid dynamics, Euler's equation for particle trajectory, Newton's second law, Laplace's equation for current density, and the Maxwell-Stefan equation for the electromagnetic field. The impact of critical operating parameters, including buffer input flow rate, pinched section width, applied voltage, frequency, and magnetic field strength, on separation efficiency has been examined across four distinct systems, both with and without the application of dielectrophoresis and magnetophoresis forces. The device exhibits optimum performance at W_p=20 μm, Q_2=1250 μl/h, f=100 kHz،, V=3 V, and M=1.5 T, achieving effective separation of platelet cells, red blood cells, white blood cells, and tumor cells. In circulation, all parties want complete fulfillment. Furthermore, an analysis of the impact of applying two forces, both individually and concurrently, reveals that while each force is effective in isolation, their simultaneous application significantly enhances the separation efficiency of the system, achieving complete separation.
