چكيده به لاتين
The detection of circulating tumor cells (CTCs), which are responsible for metastasis in various types of cancer, is an important goal in oncological diagnosis and treatment. In fact, CTCs are a vital component in cancer diagnosis and monitoring. These cells disseminated in the bloodstream information They are valuable regarding the progression of the disease and the effectiveness of the treatment.The detection of these cells is very challenging in our design, despite the low concentration (1-10 cells in 7.5 ml of blood). For the first time, we propose surface plasmon resonance (SPR) as a powerful technique for the label-free detection of biomolecules and analytes in order to detect CTCs using surface plasmon stimulation, which is one of the methods to investigate cancer progression Chemical has emerged.
This thesis focuses on the development and validation of a plasmonic sensor for the detection of HER2-positive SKBR3 cells. The limit of detection (LOD) obtained was 1 cell in 250 μl. We incubated different concentrations of SK-BR3 breast cancer cells, 1, 5, 50, 100, and 250 cells in PBS medium on the surface of five sensors. Changes in the resonance wavelength of each of the 5 prisms were observed before and after the addition of cancer cells, with observed changes of 7 nm for one cell, 9 nm for five cells, 29 nm for fifty cells, 45 nm for one hundred cells, and 42 nm was obtained for 250 cancer cells. In addition, in a parallel experiment, we added 1, 5, 50, 100, and 250 SK-BR3 cells stained with DAPI in the complex medium of human blood and incubated on the surface of 5 sensors and counted using a fluorescence microscope, and the cells We counted 1, 5, 43, 91 and 217, respectively, these results were obtained without using Ficoll in the complex environment of human blood. came Blood samples These findings highlight the potential of the sensor for sensitive detection and quantification of circulating tumor cells in clinical diagnostics.
