Design an‎d Fabrication of an Electrochemical Nanobiosensor Based on Covalent Organic Framework (COF) Nanopolymer an‎d Gold Nanoparticles on a Graphite Electrode Substrate for the Detection of SKBR-3 Cell Line in Breast Cancer (BC)

1/5/2025 12:00:00 AM

In this research, a novel electrochemical sensor was developed for the specific detection of HER2+ SKBR3 breast cancer cells. The sensor utilizes a modified graphite electrode functionalized with a covalent organic framework (COF) polymer, gold nanoparticles, an‎d graphene oxide. The COF polymer serves as a stable platform for immobilizing gold nanoparticles, thereby increasing the electrodeʹs specific surface area. Gold nanoparticles act as a signal amplifier, enhancing the sensorʹs sensitivity. Antibodies specific to the HER2 receptor are immobilized on the surface of gold nanoparticles. The interaction between the antibodies an‎d HER2 receptors on the surface of SKBR3 cells generates a measurable electrochemical signal. Results demonstrate that this sensor exhibits high sensitivity an‎d selec‎tivity for the detection of SKBR3 cancer cells, making it a promising diagnostic tool for the early detection of breast cancer. Within the linear range of 1 to 300 cells, the limit of detection (LOD) of this nanosensor is as low as one cell. This indicates that the sensor is capable of accurately detecting the presence of even a single cell.

سنسور الكتروشيميايي , سلول‌هاي سرطانيSKBR3 , گيرنده‌ي HER2 , نانو ذرات طلا , نانو پليمر چهارچوب آلي كوالانسي، , الكترود گرافيتي , تشخيص سرطان سينه

Electrochemical sensor , SKBR3 cancer cells , HER2 receptor , gold nanoparticles , covalent organic framework , breast cancer detection , graphite electrode

Nahid Shanehnand

Dr. Meysam Jalali